FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3668887
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT202400.5516659411968807No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT154440.42094509860892415RNA PCR Primer, Index 39 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTT129880.3540038164162592TruSeq Adapter, Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGT115820.31568156773430195No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT90790.24745924308925293RNA PCR Primer, Index 39 (96% over 25bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTTCT72780.1983707865627914RNA PCR Primer, Index 11 (95% over 23bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT50020.13633562440053346RNA PCR Primer, Index 39 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45160.12308910031843445No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)