FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103814
Sequences flagged as poor quality0
Sequence length101
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT98069.445739495636426No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT69836.726453079546111TruSeq Adapter, Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGT50254.840387616313792No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTT47894.6130579690600495No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT33893.264492265012426RNA PCR Primer, Index 10 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCT20952.018032249985551Illumina PCR Primer Index 5 (95% over 21bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT14501.3967287649064675TruSeq Adapter, Index 10 (96% over 26bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG14381.385169630300345TruSeq Adapter, Index 10 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCT11151.0740362571522146Illumina PCR Primer Index 5 (95% over 24bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCTTG10971.0566975552430307Illumina PCR Primer Index 5 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC10481.009497755601364No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA8650.8332209528579959RNA PCR Primer, Index 10 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT7310.7041439497562949No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTC6850.6598339337661587No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG6690.6444217542913288TruSeq Adapter, Index 10 (95% over 24bp)
CTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCTTGA6030.5808465139576551Illumina PCR Primer Index 5 (96% over 26bp)
CACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTG4910.4729612576338451Illumina PCR Primer Index 5 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCT4530.4363573313811239No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC2550.24563161038010287Illumina Paired End PCR Primer 2 (95% over 24bp)
ACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGC1460.1406361377078236Illumina PCR Primer Index 5 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCG1350.130040264318878RNA PCR Primer, Index 10 (95% over 22bp)
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTT1100.10595873388945613TruSeq Adapter, Index 10 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)