FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005110794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74425733
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATGGCTAGTGTAGATCTC763660.10260698406557851Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG648050.018.610489
GAGCGTC745650.016.1939478
AGAGCGT799900.015.1415137
ATCGGAA904500.013.5608981
CGCCGTA187000.013.415091555-59
TCGGAAG919050.013.3541542
AAGAGCG927800.013.21826656
CGGAAGA957300.012.84804253
TCGCCGT213450.011.86822955-59
GCCGTAT218400.011.559455-59
GTCGCCG246350.010.39506755-59
CGTATCA248500.010.07667860-64
TGGTCGC264150.09.84720250-54
CCGTATC249000.09.76613855-59
GTGGTCG287300.09.14457750-54
GAAGAGC1397850.09.0740045
GGTCGCC282150.08.76677850-54
TCTCGGT360600.07.466675345-49
TCGGTGG367000.06.98090345-49
CTCGGTG405000.06.78772845-49
Produced by FastQC (version 0.11.5)