FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005110799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81268540
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA369250.014.0263331
TCGGAAG391750.013.2335852
CGGAAGA422400.012.3551943
TATGCCG134600.09.85857145-49
CTCGTAT159900.08.515408540-44
CGTATGC161600.08.22935845-49
TCTCGTA159400.07.85984140-44
TCGATCA171150.07.590674435-39
ATGCCGT185100.07.231580745-49
CGATCAT189700.06.634400435-39
GCCGTCT199500.06.62991150-54
AGAGCAC881050.06.5324837
ATCTCGT205250.06.26655440-44
TCGTATG184200.06.093118740-44
AGCACAC943100.05.98735959
ACACTCG230000.05.982175430-34
GAGCACA966350.05.97836168
ACTCGAT246000.05.705070530-34
GAAGAGC998500.05.69852645
TGCCGTC196850.05.510666445-49
Produced by FastQC (version 0.11.5)