FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005110805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673657
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT48430.7189118497989333TruSeq Adapter, Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT43290.6426118929959905No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTT32640.48451957004825896No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT28770.4270719372024636RNA PCR Primer, Index 10 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGT22730.33741206578421956No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCT17810.2643778658872393Illumina PCR Primer Index 5 (95% over 21bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT16770.24893974233178012TruSeq Adapter, Index 10 (96% over 26bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG16220.2407753500668738TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCTTG12300.18258549974245053Illumina PCR Primer Index 5 (96% over 26bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCT11150.16551449773401006Illumina PCR Primer Index 5 (95% over 24bp)
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG6780.10064469010193616TruSeq Adapter, Index 10 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)