FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217814
Sequences flagged as poor quality0
Sequence length101
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT203229.329978789242197No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT169497.781409826732901TruSeq Adapter, Index 10 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTT110065.052935073043973No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGT100964.635147419357801No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT81243.7297878006005125RNA PCR Primer, Index 10 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCT49142.256053329905332Illumina PCR Primer Index 5 (95% over 21bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG39381.8079645936441184TruSeq Adapter, Index 10 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT35651.636717566363962TruSeq Adapter, Index 10 (96% over 26bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCTTG29841.3699762182412516Illumina PCR Primer Index 5 (96% over 26bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCT24801.1385861331227562Illumina PCR Primer Index 5 (95% over 24bp)
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA21770.9994766176646128RNA PCR Primer, Index 10 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC19660.90260497488683No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG15820.7263077671775001TruSeq Adapter, Index 10 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT15350.704729723525577No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCTTGA14740.6767241775092511Illumina PCR Primer Index 5 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTC12530.5752614616140377No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTG11620.5334826962454204Illumina PCR Primer Index 5 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCT8800.40401443433388123No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC7150.32826172789627844Illumina Paired End PCR Primer 2 (95% over 24bp)
ACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGC4010.18410203200896177Illumina PCR Primer Index 5 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCG3330.1528827348104346RNA PCR Primer, Index 10 (95% over 22bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGAGGCT2880.13222290578199747No Hit
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTT2800.1285500472880531TruSeq Adapter, Index 10 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCG2530.11615414987099085No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)