FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005110813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53641191
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA239700.028.7619321
TCGGAAG248400.027.5275462
CGGAAGA269700.025.3784333
TATGCCG105500.013.49631745-49
ACCGATC103050.012.39696235-39
AGAGCAC578100.012.3771427
CGTATGC121000.011.82736545-49
AGCACAC618100.011.7639199
CTCGTAT122250.011.68249640-44
AACCGAT113500.011.49830935-39
GAGCACA629450.011.4941148
GAAGAGC645700.011.3508035
TCTCGTA115450.011.15237440-44
CCGATCT117650.010.94481435-39
CAACCGA131050.010.93219235-39
TGCCGTC138550.010.30827445-49
TCGTATG138850.010.27537240-44
ATGCCGT141850.010.23201145-49
ACAACCG139300.010.05541530-34
GCCGTCT144400.09.75977250-54
Produced by FastQC (version 0.11.5)