FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62850
Sequences flagged as poor quality0
Sequence length101
%GC69

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT1174718.690533015115353No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT885814.093874303898172TruSeq Adapter, Index 10 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTT54788.71599045346062No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGT53828.563245823389023No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT41736.639618138424821RNA PCR Primer, Index 10 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCT25994.135242641209228Illumina PCR Primer Index 5 (95% over 21bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT18812.9928400954653935TruSeq Adapter, Index 10 (96% over 26bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG18002.863961813842482TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCTTG12932.0572792362768495Illumina PCR Primer Index 5 (96% over 26bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCT12181.9379474940334127Illumina PCR Primer Index 5 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC11161.775656324582339No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA9511.513126491646778RNA PCR Primer, Index 10 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT9081.4447096260938743No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG8131.2935560859188544TruSeq Adapter, Index 10 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTC6951.105807478122514No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTG6010.9562450278440733Illumina PCR Primer Index 5 (95% over 22bp)
CTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCTTGA5950.9466984884645983Illumina PCR Primer Index 5 (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCT5190.8257756563245823No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC3610.5743834526650756Illumina Paired End PCR Primer 2 (95% over 24bp)
ACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGC2010.31980906921241053Illumina PCR Primer Index 5 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCG1680.26730310262529833RNA PCR Primer, Index 10 (95% over 22bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGAGGCT1410.2243436754176611No Hit
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTT1320.2100238663484487TruSeq Adapter, Index 10 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCG1220.19411296738265713No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCGT1000.15910898965791567No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCGT900.1431980906921241No Hit
CTTTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC870.13842482100238665No Hit
ATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCTT830.13206046141607Illumina PCR Primer Index 5 (96% over 25bp)
TCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG750.11933174224343676No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCGGCT730.11614956245027844No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTG660.10501193317422435No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCGGCTTG660.10501193317422435Illumina PCR Primer Index 5 (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)