FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005110831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162056
Sequences flagged as poor quality0
Sequence length101
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT2029212.521597472478648No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT1735910.711729278767832TruSeq Adapter, Index 10 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTT113146.981537246383966No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGT97636.024460680258676No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT83165.131559460927087RNA PCR Primer, Index 10 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCT50663.1260798736239326Illumina PCR Primer Index 5 (95% over 21bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG39672.4479192377943426TruSeq Adapter, Index 10 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT35942.2177518882361653TruSeq Adapter, Index 10 (96% over 26bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCTTG31181.924026262526534Illumina PCR Primer Index 5 (96% over 26bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCT26211.6173421533297132Illumina PCR Primer Index 5 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC22051.3606407661549094No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA21841.3476822826677197RNA PCR Primer, Index 10 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG16651.0274226193414622TruSeq Adapter, Index 10 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT16100.9934837340178703No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCTTGA14820.9144986918102385Illumina PCR Primer Index 5 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTC14230.8780915239176581No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTG12470.7694870908821642Illumina PCR Primer Index 5 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCT9380.5788122624278027No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC7260.4479932862714124Illumina Paired End PCR Primer 2 (95% over 24bp)
ACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGC4310.26595744680851063Illumina PCR Primer Index 5 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCG3340.20610159451053955RNA PCR Primer, Index 10 (95% over 22bp)
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTT2710.1672261440489707TruSeq Adapter, Index 10 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCG2710.1672261440489707No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGAGGCT2440.15056523670829838No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCGT2180.13452140000987314No Hit
ATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCTT2040.12588241101841338Illumina PCR Primer Index 5 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCGT2030.12526534037616627No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCGGCTTG1820.11230685688897665Illumina PCR Primer Index 5 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)