FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500566
Sequences flagged as poor quality0
Sequence length101
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT468429.35780696251843TruSeq Adapter, Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT436768.725322934438216No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTT311856.229947699204501No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT231774.6301586603964315RNA PCR Primer, Index 10 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGT216214.31931054046819No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG148032.957252390294187TruSeq Adapter, Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCT146592.928484955030905Illumina PCR Primer Index 5 (95% over 21bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCTTG116182.3209726589500685Illumina PCR Primer Index 5 (96% over 26bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT115322.3037921073344974TruSeq Adapter, Index 10 (96% over 26bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCT82291.6439390609829674Illumina PCR Primer Index 5 (95% over 24bp)
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA76131.5208783656900389RNA PCR Primer, Index 10 (96% over 28bp)
CTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCTTGA54371.0861704550448892Illumina PCR Primer Index 5 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC52991.0586016629175772No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG51101.0208444041345197TruSeq Adapter, Index 10 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT41590.8308594670832617No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTG37360.746355125997371Illumina PCR Primer Index 5 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTC34480.688820255470807No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC26110.5216095380029807Illumina Paired End PCR Primer 2 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCT24040.48025634981201276No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGC16330.3262307068398573Illumina PCR Primer Index 5 (95% over 23bp)
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTT9410.18798719849130785TruSeq Adapter, Index 10 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCG9140.1825933043794425RNA PCR Primer, Index 10 (95% over 22bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGAGGCT9030.1803957919634973No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCG7360.14703355801233003No Hit
ATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCTGCTT7220.14423672402839985Illumina PCR Primer Index 5 (96% over 25bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCGGCTTG6060.12106295673297827Illumina PCR Primer Index 5 (96% over 26bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCTTCGGCTTG6000.11986431359700819No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGTCGT5830.11646815804509296No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCGT5410.10807765609330239No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)