FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005110869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1739813
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT74800.42993126272766097No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT42020.24152020935583307RNA PCR Primer, Index 33 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCGCGTATGCCGT33550.192836816370495No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCGCGTATGCCGTCTT22870.13145090880456692No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20950.12041524002866974No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)