FastQCFastQC Report
Sun 7 Aug 2022
EGAF00005110871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4077332
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT100570.2466563919739673No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC57250.14041044486934104No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48410.11872960063100085No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT48110.11799382537404361RNA PCR Primer, Index 33 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCGCGTATGCCGT42270.1036707337052759No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)