FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758253
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT96060.5463377568529671No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT45370.25804022515531044RNA PCR Primer, Index 33 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCGCGTATGCCGT40820.2321622656125142No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCGCGTATGCCGTCTT25750.146452188621319No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22000.12512419998714633No Hit

[FAIL]Adapter Content

Adapter graph