FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005110899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125418326
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCAAATCTCGTATG1502100.1197671861766039TruSeq Adapter, Index 18 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1065350.023.9939231
TCGGAAG1093750.023.380672
CGGAAGA1129350.022.611553
TATGCCG442350.018.03226545-49
CTCGTAT479500.016.62262540-44
CGTATGC484450.016.26467145-49
TCTCGTA456200.016.2511140-44
GATCGGA860600.015.814821
ATGCCGT513450.015.47876545-49
AGAGCAC1781900.014.7884597
GCCGTCT533700.014.61528250-54
AGCACAC1860700.014.1816539
GAGCACA1899200.013.8941678
ATCTCGT534650.013.80689640-44
GAAGAGC1974000.013.3199865
CGTCTTC603650.013.25079350-54
CCGTCTT615400.012.87290550-54
GTCACGT661750.012.1321625-29
AGTCACG676850.011.92576825-29
CGTCTGC700050.011.51794130-34
Produced by FastQC (version 0.11.5)