FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005110901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51689036
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTATCATCTCGTATG544430.10532794614316274TruSeq Adapter, Index 13 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA324550.028.9506781
TCGGAAG335500.028.2621082
CGGAAGA359200.026.0926843
GATCGGA244050.024.1405851
TATGCCG166500.018.73462945-49
CTCGTAT186450.016.71237840-44
CGTATGC183000.016.66506845-49
TCTCGTA176850.016.22575640-44
ATGCCGT193650.015.88334845-49
GCCGTCT200650.014.73722150-54
AGAGCAC653300.014.6905267
AGCACAC674200.014.4179019
ATCTCGT205900.013.85903540-44
GAGCACA697850.013.7942488
GAAGAGC714650.013.5509475
CGTCTTC231250.013.20103150-54
CCGTCTT233400.012.95515850-54
CATCTCG235450.012.00881440-44
ACACGTC284600.011.06913110-14
CGTCTGA279900.010.98570915-19
Produced by FastQC (version 0.11.5)