FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005110902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51689036
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATAGGCTGTGTAGATCTC593790.11487736006529509Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG259450.035.1408849
GAGCGTC309350.029.6346598
ATCGGAA345600.026.8204921
AGAGCGT342550.026.7619977
TCGGAAG360050.025.659022
CGGAAGA390800.023.6766533
AAGAGCG399800.023.1618656
GATCGGA266000.021.7756771
CGCCGTA152250.019.49050555-59
TCGCCGT168800.017.60501155-59
GCCGTAT178050.016.81105455-59
GTCGCCG185050.015.99712855-59
TGGTCGC203900.014.87665150-54
CGTATCA205200.014.50283960-64
GTGGTCG215700.014.07609450-54
GAAGAGC734850.013.1472985
TCTCGGT249250.012.43984745-49
GCGTCGT246000.012.11733410-14
CGTCGTG250000.011.95783510-14
CTCGGTG272200.011.34365445-49
Produced by FastQC (version 0.11.5)