FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005110923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76491112
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA334900.038.491611
ATCGGAA381150.033.9731862
TCGGAAG405700.032.058483
CGGAAGA441100.029.4683424
TATGCCG192450.016.36445445-49
AGAGCAC886600.015.0447398
CTCGTAT212000.014.95709540-44
CGTATGC220100.014.43384445-49
TCTCGTA208850.014.1413540-44
GAGCACA960250.013.8757289
GAAGAGC991250.013.6613046
ATGCCGT235300.013.40284345-49
GCCGTCT250400.012.47968750-54
ATCTCGT252700.011.85958740-44
TCGTATG231500.011.36803245-49
CCGTCTT295450.010.7093150-54
CGTCTTC297500.010.65988650-54
TGCCGTC245350.010.62660150-54
AAGAGCA1392600.010.0468397
CGTCTGA344500.09.28833115-19
Produced by FastQC (version 0.11.5)