FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005110958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53571429
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATTACCGGTGTAGATCTC549540.1025807991793536Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG190000.043.804839
GAGCGTC238500.035.1092578
ATCGGAA268300.031.5359151
AGAGCGT270950.031.0375277
TCGGAAG290350.029.1906262
CGGAAGA319100.026.4690323
AAGAGCG335350.025.3581756
CGCCGTA101800.017.21895655-59
TCGCCGT115600.015.25119955-59
GTCGCCG131100.013.65817155-59
GCCGTAT132100.013.51087655-59
GAAGAGC702200.012.6368515
TACCGGT143250.012.47960835-39
CGGTGTA146300.012.37800335-39
TGGTCGC145350.012.3490850-54
CCGTATC146050.011.72402155-59
GTGGTCG157550.011.56766950-54
ACCGGTG156200.011.56563535-39
GGTCGCC154150.011.3337250-54
CGTATCA160350.011.08533260-64
Produced by FastQC (version 0.11.5)