FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005110965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58902676
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACGTCCAAATCTCGTATG852910.14479987292937252TruSeq Adapter, Index 16 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA575000.025.861521
TCGGAAG589750.025.1624982
CGGAAGA612150.024.2180183
TATGCCG232850.018.61532645-49
AGAGCAC892400.017.0427577
CTCGTAT254650.017.00224740-44
CGTATGC253800.016.85016445-49
AGCACAC930350.016.3631489
TCTCGTA245500.016.29522740-44
GAGCACA951450.016.0459828
ATGCCGT266700.015.98620245-49
GAAGAGC988850.015.3877935
GCCGTCT278950.014.96000550-54
GATCGGA463250.014.372951
ATCTCGT279550.014.37266340-44
CGTCTTC308900.013.78178550-54
CCGTCTT316200.013.30310850-54
ACGTCCA333300.012.97683530-34
AAGAGCA1275700.012.1948126
CACGTCC380100.011.54308530-34
Produced by FastQC (version 0.11.5)