FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005110979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67748315
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTGCATATCTCGTATG950560.14030754860840453TruSeq Adapter, Index 16 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTGCATATCTCGTAT794290.11724129227420638TruSeq Adapter, Index 16 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA309050.040.936231
ATCGGAA437500.037.0654221
TCGGAAG455500.035.6882132
CGGAAGA488100.033.353223
TATGCCG274950.021.34088945-49
CTCGTAT293650.019.91039740-44
TCTCGTA282700.019.37361140-44
CGTATGC298250.019.2895545-49
AGAGCAC879550.018.93037
ATGCCGT313350.018.48496845-49
TCGTGCA321550.018.22033930-34
AGCACAC923750.018.12659
TATCTCG298750.017.90565540-44
GAGCACA951100.017.5061998
GCCGTCT321000.017.39914350-54
GAAGAGC982100.017.0487735
ATCTCGT322050.016.8398340-44
ACTCGTG358500.016.20489530-34
CACTCGT355450.016.19302730-34
CTCGTGC375300.015.76150330-34
Produced by FastQC (version 0.11.5)