FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005110994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84012550
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACGTCTGGTGTAGATCTC1353710.1611318785110082Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG528700.040.934649
GAGCGTC613100.035.5334478
AGAGCGT678400.032.1336447
ATCGGAA717650.030.3809241
TCGGAAG740200.029.4976232
AAGAGCG783000.027.9772286
CGGAAGA794300.027.5605773
CGCCGTA260250.020.1502855-59
TCGCCGT289400.018.2641355-59
GCCGTAT300050.017.67790655-59
GTCGCCG312100.016.95407955-59
GAAGAGC1339500.016.744475
TGGTCGC341700.015.66590250-54
CGTATCA346100.015.28830160-64
GTGGTCG364650.014.75062750-54
TAACGTC393800.013.60399530-34
GTAACGT398750.013.28069130-34
CCGTATC339050.012.86529255-59
TCTCGGT427350.012.82420945-49
GGTCGCC352300.012.42229550-54
Produced by FastQC (version 0.11.5)