FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2787103
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT158920.5701978003683394No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT84010.30142409519849106TruSeq Adapter, Index 1 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGT79210.2842019114471191No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGTCTT56770.20368820240945526No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCT39460.1415807022560702RNA PCR Primer, Index 1 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)