FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005111026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68515913
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG232850.023.5702789
GAGCGTC301400.018.3538688
AGAGCGT349150.016.113737
ATCGGAA357000.015.7526231
TCGGAAG375550.015.0053482
CGGAAGA414700.013.4966143
AAGAGCG433950.012.9650756
CGCCGTA99150.012.03509855-59
TCGCCGT118400.010.20083655-59
GTCGCCG137900.08.87402555-59
GCCGTAT140450.08.82646755-59
TGGTCGC161450.07.768007350-54
CGTATCA167700.07.57403660-64
CCGTATC161150.07.386782655-59
GTGGTCG180700.07.092929450-54
GGTCGCC167350.07.00030850-54
GAAGAGC886500.06.8536435
TCTCGGT234600.05.64282145-49
GCGTCGT216850.05.556792310-14
CGTCGTG221000.05.426210-14
Produced by FastQC (version 0.11.5)