FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005111031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81850040
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA463350.012.8537171
TCGGAAG490450.012.15080452
CGGAAGA523500.011.4369283
TATGCCG143600.09.069445-49
CTCGTAT168800.07.903359440-44
CGTATGC172900.07.607972645-49
TCTCGTA168450.07.317188740-44
ACCGGTT185100.07.09755730-34
CCGGTTA173850.07.031369730-34
CGGTTAA185850.06.998715435-39
ATGCCGT196700.06.84964445-49
AGAGCAC959550.06.73807437
TCGTATG191300.06.632685740-44
CACCGGT205000.06.44394130-34
AGCACAC1026800.06.3320629
TGCCGTC206150.06.28238845-49
GAGCACA1042950.06.26875978
GCCGTCT217500.06.19469450-54
GAAGAGC1069100.06.1696625
ATCTCGT220550.05.77276440-44
Produced by FastQC (version 0.11.5)