FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005111033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966243
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT66250.6856453293840162No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT63650.6587369843817756TruSeq Adapter, Index 1 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGTCTT43860.4539230814608748No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGT35850.3710246801270488No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCT34270.3546726858564564RNA PCR Primer, Index 1 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGTCTTCT20250.20957461011360495No Hit
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCT20250.20957461011360495TruSeq Adapter, Index 1 (96% over 27bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGTCTTCTGCT12200.126262234241283Illumina Paired End PCR Primer 2 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)