FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005111035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2249750
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT104880.46618513168129794No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT72580.3226136237359707TruSeq Adapter, Index 1 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGT49050.21802422491387932No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGTCTT46080.2048227580842316No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCT37980.16881875763973775RNA PCR Primer, Index 1 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)