FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005111037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73045906
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTAGTGATCTCGTATG2231290.30546407351015675TruSeq Adapter, Index 11 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA703400.057.00151
TCGGAAG721100.055.408612
CGGAAGA751800.053.115933
AGAGCAC1153850.034.813857
AGCACAC1204050.033.6026579
GAGCACA1218150.033.136898
GAAGAGC1256050.032.108165
AAGAGCA1629050.024.929986
GGAAGAG1835800.022.2291564
TATGCCG413300.022.0101845-49
CTCGTAT434150.020.87299340-44
CGTATGC437200.020.35594245-49
TCTCGTA411450.020.09345640-44
ATGCCGT453250.019.77267345-49
GCCGTCT457200.018.96163250-54
ATCTCGT455200.017.99342340-44
TCGTATG464250.017.91125340-44
TGCCGTC458050.017.8118345-49
CGTCTTC508300.017.5831250-54
CCGTCTT513100.017.1049650-54
Produced by FastQC (version 0.11.5)