FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005111042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48755089
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATGTGTGGTGTAGATCTC607340.1245695603181034Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG288750.032.5578589
GAGCGTC338000.027.9639648
AGAGCGT373400.025.4691937
ATCGGAA399850.023.6791
TCGGAAG413650.022.8534562
AAGAGCG437200.021.8519886
CGGAAGA438700.021.594793
CGCCGTA135800.018.02369755-59
TCGCCGT149000.016.47562455-59
GCCGTAT158700.015.52342955-59
GTCGCCG166500.014.7613755-59
TGGTCGC182900.013.753674550-54
CGTATCA181400.013.5722160-64
GTGGTCG194950.012.93329950-54
GAAGAGC770450.012.6540225
CCGTATC175100.010.88957255-59
TCTCGGT240250.010.80890745-49
GGTCGCC188850.010.18027450-54
CTCGGTG259150.010.08774445-49
CGGTGGT254350.010.06681250-54
Produced by FastQC (version 0.11.5)