FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806435
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT51350.6367531171142126No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT44510.5519353698686192TruSeq Adapter, Index 1 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGTCTT27590.34212304773478336No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGT25560.31695052918090116No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCT22630.28061778072628296RNA PCR Primer, Index 1 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGTCTTCT13200.16368337187746068No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTG11870.147191032135262TruSeq Adapter, Index 1 (96% over 29bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCT11580.14359495805613595TruSeq Adapter, Index 1 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8730.10825423003713876No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)