FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175780
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT109860.5049223726663541No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT57590.2646866870731416Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGT51020.23449061945601118No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTT37730.1734090762852862No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT30950.14224783755710596Illumina PCR Primer Index 10 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24390.11209773046907316No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)