FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005111085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65874325
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCCTACATCTCGTATG1616510.24539302679761804TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA591150.048.0744321
TCGGAAG610050.046.4890862
CGGAAGA632650.044.862443
AGAGCAC987750.028.9176627
AGCACAC1028200.027.8574989
GAGCACA1048950.027.3618018
GAAGAGC1083450.026.6109495
TATGCCG300100.020.67878745-49
AAGAGCA1408550.020.6337326
CTCGTAT320250.019.37750240-44
CGTATGC318000.019.07251745-49
TCTCGTA300150.018.49652940-44
GGAAGAG1627350.018.1356774
ATGCCGT335150.018.10088245-49
TCGTATG339100.017.27842940-44
GCCGTCT342250.016.92899950-54
TGCCGTC344200.016.61396645-49
ATCTCGT339200.016.12776440-44
CGTCTTC379350.015.72437750-54
CCGTCTT389150.015.1085650-54
Produced by FastQC (version 0.11.5)