FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1876010
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT39200.20895411005271827No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT36260.1932825517987644Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT24300.12953022638472075Illumina PCR Primer Index 10 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTT24290.12947692176480938No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21860.11652389912633729No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGT19700.1050101012254732No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)