FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2832575
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT163980.5789078841689982No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT121730.4297503155256259Illumina PCR Primer Index 10 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTT77250.27272005154320716No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGT75190.26544751683538825No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT70080.24740739433201242Illumina PCR Primer Index 10 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTTCT41530.1466157118522899RNA PCR Primer, Index 12 (95% over 21bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT31940.11275959153773511Illumina PCR Primer Index 10 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30060.10612252102768682No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)