FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940919
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT59470.6320416528946701No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT49680.5279944394788499Illumina PCR Primer Index 10 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTT31460.3343539667070173No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT29150.3098035006201384Illumina PCR Primer Index 10 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGT27860.29609350007811513No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTTCT17050.18120582111743944RNA PCR Primer, Index 12 (95% over 21bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT14530.1544234944772079Illumina PCR Primer Index 10 (96% over 26bp)
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCTTG13710.14570861041173575Illumina PCR Primer Index 10 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13150.1397569822694621No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTTCTGCT9540.10139023656659074TruSeq Adapter, Index 12 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)