FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005111107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4414383
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT244330.5534861836863725No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT195530.4429384582171506Illumina PCR Primer Index 10 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTT133490.3023978662476727No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGT120140.2721558143006622No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT119920.27165744340715337Illumina PCR Primer Index 10 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTTCT74360.16844936200597002RNA PCR Primer, Index 12 (95% over 21bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCTGCT63070.14287387387999637Illumina PCR Primer Index 10 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52280.11843104687563359No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)