FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005111125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77116783
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACCGGTTAATCTCGTATG837420.1085911480513911TruSeq Adapter, Index 5 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA443250.034.4187161
TCGGAAG465650.032.6284262
CGGAAGA494400.030.9802683
AGAGCAC925800.016.8966087
TATGCCG193850.016.60031545-49
AGCACAC975100.016.1389759
GAGCACA999350.015.6530548
GAAGAGC1035700.015.1876165
CTCGTAT217500.014.84549340-44
CGTATGC218650.014.63786345-49
CCGGTTA209650.014.5912430-34
ACCGGTT223850.014.31330130-34
CGGTTAA218850.014.25623535-39
TCTCGTA211800.013.93067540-44
CACCGGT236200.013.69994730-34
ATGCCGT238550.013.453238545-49
TCGTATG239100.013.14053340-44
TGCCGTC248850.012.38940445-49
GCCGTCT249400.012.27280750-54
ATCTCGT255750.011.559440-44
Produced by FastQC (version 0.11.5)