FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2242566
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT75320.3358652543559476No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT57360.25577842525036054TruSeq Adapter, Index 11 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTT39010.1739525168935942Illumina PCR Primer Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGT34860.15544692999002035No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT34290.1529051987767584RNA PCR Primer, Index 11 (96% over 25bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTTCT23430.10447853039776756Illumina PCR Primer Index 11 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)