FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005111147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1701837
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT78110.45897462565451336TruSeq Adapter, Index 11 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT76160.4475164190225033No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTT53240.3128384210708781Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT49640.29168480882716735RNA PCR Primer, Index 11 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGT35580.20906820100867474No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTTCT31630.1858579875746032Illumina PCR Primer Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT28870.1696402181877583TruSeq Adapter, Index 11 (96% over 28bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTTCTGCT21270.12498259233992444Illumina PCR Primer Index 11 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)