FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005111151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47706397
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTACACACGATCTCGTATG824030.17272945596792816TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA450050.036.6175961
TCGGAAG459950.035.6691132
CGGAAGA482650.033.9614833
AGAGCAC721450.023.0521777
AGCACAC757200.022.174289
GAGCACA768100.021.8218568
GAAGAGC799600.020.889945
TATGCCG197200.019.15412545-49
CTCGTAT209050.017.82591640-44
CGTATGC212850.017.01689545-49
ATGCCGT221050.016.71361545-49
AAGAGCA1037350.016.2767986
TCTCGTA187600.016.26229340-44
GCCGTCT224400.015.72028950-54
TCGTATG225150.015.23742940-44
TGCCGTC223100.015.14320845-49
CGTCTTC252850.014.63386450-54
GGAAGAG1195050.014.261914
CCGTCTT253000.014.19537350-54
ATCTCGT217100.013.73194940-44
Produced by FastQC (version 0.11.5)