FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005111152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47706397
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGTGTGTAGTGTAGATCTC1042620.21854930691999228Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG355400.045.672749
GAGCGTC403550.040.402868
AGAGCGT438200.037.3890727
ATCGGAA469600.035.1298031
TCGGAAG481450.034.196622
AAGAGCG496950.033.100746
CGGAAGA499550.032.943013
CGCCGTA170050.021.36737655-59
GAAGAGC816000.020.5228525
TCGCCGT184900.019.63560155-59
GCCGTAT193950.018.80160955-59
GTCGCCG193650.018.77832455-59
TGGTCGC212500.017.2355850-54
CGTATCA213350.017.06558460-64
GTGGTCG220750.016.66369450-54
CCGTATC209250.015.81929955-59
GGTCGCC216350.015.44105850-54
TCTCGGT262550.014.23290745-49
GGAAGAG1207100.014.1977834
GTGTCGT266200.013.74803125-29
Produced by FastQC (version 0.11.5)