FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005111175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2414905
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT93940.3890008095556554No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT64990.26912031736238073TruSeq Adapter, Index 11 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGT43280.17922030059153465No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTT43010.17810224418765955Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT41460.1716837722394877RNA PCR Primer, Index 11 (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29810.12344170888709909No Hit
ACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTTCT25910.10729200527556984Illumina PCR Primer Index 11 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)