FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005111197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1570787
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT78100.49720299442254107No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT60190.38318371618812735TruSeq Adapter, Index 12 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGT40230.25611365512956247No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTT39740.2529941997228141No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT35490.22593769874591527RNA PCR Primer, Index 12 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCT21070.13413658249017849Illumina PCR Primer Index 12 (95% over 22bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCT20000.12732471047952396TruSeq Adapter, Index 12 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19350.12318665738893941No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)