FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005111200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65629136
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG360800.021.249839
GAGCGTC434800.017.5499218
AGAGCGT476850.016.2613227
ATCGGAA517200.015.0490481
TCGGAAG534800.014.4286162
AAGAGCG573100.013.6567456
CGGAAGA572800.013.4205373
CGCCGTA127950.012.27822555-59
TCGCCGT149150.010.63989855-59
GCCGTAT161150.09.98249855-59
GTCGCCG172500.09.30887555-59
CCGTATC183800.08.68137355-59
CGTATCA189550.08.63902960-64
TGGTCGC193450.08.56272250-54
GAAGAGC990800.08.2716625
GTGGTCG207000.08.03722150-54
GGTCGCC203150.07.889921750-54
TCTCGGT262650.06.48814545-49
TCGGTGG263550.06.356022445-49
CGGTGGT291450.05.852572450-54
Produced by FastQC (version 0.11.5)