FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005111201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473160
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT119800.8132178446333053No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT65590.4452333758722745TruSeq Adapter, Index 12 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGT54100.3672377745798148No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTT42680.2897173423117652No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT33020.22414401694316977RNA PCR Primer, Index 12 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20260.13752749192212657No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCT19290.13094300686958646Illumina PCR Primer Index 12 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)