FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000530
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT68990.34485861246769606No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT60270.3012701634066972TruSeq Adapter, Index 12 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTT38550.1926989347822827No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT38130.19059949113484925RNA PCR Primer, Index 12 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGT36160.18075210069331626No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCT21170.10582195718134695Illumina PCR Primer Index 12 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)