FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88338
Sequences flagged as poor quality0
Sequence length101
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT1054611.938237225203196No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT71148.053159455726867TruSeq Adapter, Index 12 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGT46615.276325024338337No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTT43314.902759854196382No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT37984.299395503633771RNA PCR Primer, Index 12 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCT21582.442889809594965Illumina PCR Primer Index 12 (95% over 22bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCT16681.8882021327175167TruSeq Adapter, Index 12 (96% over 27bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCTGCT9881.1184314790916705Illumina PCR Primer Index 12 (96% over 25bp)
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC8780.993909755711019No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTG8710.9859856460413412TruSeq Adapter, Index 12 (96% over 29bp)
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTG7890.8931603613394009TruSeq Adapter, Index 12 (96% over 25bp)
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT6410.7256220426090697RNA PCR Primer, Index 12 (95% over 21bp)
TCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCTGCTTG5080.575063958885191Illumina PCR Primer Index 12 (96% over 27bp)
CACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCTG5010.5671398492155131Illumina PCR Primer Index 12 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTC4400.4980868935226064No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCT3660.4143177341574407No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGCTTGA3630.41092168715615024RNA PCR Primer, Index 12 (96% over 29bp)
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCTGC2910.32941655912517825Illumina PCR Primer Index 5 (96% over 25bp)
CTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCTGCTTGA2110.23885530575743166Illumina PCR Primer Index 12 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCG1930.2184790237496887RNA PCR Primer, Index 12 (95% over 23bp)
ACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCTGC1650.18678258507097736Illumina PCR Primer Index 12 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTTCG1550.17546242840000906Illumina PCR Primer Index 12 (95% over 21bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGCGTAGT1360.15395413072516922No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCGT1230.1392379270529104No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.12225769204645794No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCGT1060.11999366071226425No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTATCTCGTATGCCGTC1010.1143335823767801No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)