FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005111242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59678805
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGACGTTAGTGTAGATCTC697910.11694436575933448Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG289600.036.9203229
GAGCGTC349250.030.8220088
AGAGCGT385150.027.8935037
ATCGGAA414250.026.0539931
TCGGAAG436800.024.8334712
AAGAGCG457350.023.8070936
CGGAAGA460000.023.637853
CGCCGTA119900.018.44116655-59
TCGCCGT139050.015.98486255-59
GCCGTAT147400.015.15803555-59
GTCGCCG151550.014.77165255-59
CGACGTT150800.014.68168730-34
TCGACGT153550.014.343204530-34
GTCGACG160900.013.64294330-34
TGGTCGC169400.013.45440850-54
GAAGAGC866200.012.9382655
CGTATCA174550.012.891026560-64
CCGTATC170850.012.8653655-59
GTGGTCG181100.012.53714850-54
GGTCGCC179150.012.35798550-54
Produced by FastQC (version 0.11.5)