FastQCFastQC Report
Sun 7 Aug 2022
EGAF00005111259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5011605
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCTCTTCGGCCCAGGGACACGACTGGAGATTAAAC99340.19821993153889822No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT92070.1837136007326994No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT82430.16447824599105476No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC68360.13640340769074977No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT55400.1105434287019827Illumina PCR Primer Index 10 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGT54740.10922648532755473No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)