FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1527233
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT93420.6116944827672005No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT84860.5556454057763288Illumina PCR Primer Index 10 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGTCTT71760.4698693650543172No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGT56730.371456090851887No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT53300.3489971733193298Illumina PCR Primer Index 10 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGTCTTCT41540.2719951703505621RNA PCR Primer, Index 38 (95% over 21bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCT28460.1863500854159123Illumina PCR Primer Index 10 (96% over 27bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGTCTTCTGCT23300.15256349227655505RNA PCR Primer, Index 38 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19760.12938431791350763No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)