FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005111269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005111269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541665
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT50300.3262706229952681No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT49380.32030304897626916Illumina PCR Primer Index 10 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGTCTT41460.268930020464887No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGT31780.2061407633954199No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT31030.20127589327123596Illumina PCR Primer Index 10 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGTCTTCT25340.1643677452624273RNA PCR Primer, Index 38 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19210.12460554011409743No Hit
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCT17340.11247579727113217Illumina PCR Primer Index 10 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)